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KMID : 0385520130260030182
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Analytical Science & Technology
2013 Volume.26 No. 3 p.182 ~ p.189
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Studies of separation and quantitation for selenium species in food
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Jang Hee-Young
Pak Yong-Nam
Min Hyung-Sik
Lee Jong-Hae
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Abstract
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The purpose of this research is to separate and quantitate selenium species in some food samples with HPLC-ICP-MS. Cation exchange chromatography showed efficient separation only for inorganic Se species while reversed phase ion pair chromatography showed good separation for both inorganic and organic Se species. C8 column (SymmetryshieldTM RP8, 3.5 μm, 4.6 × 150 mm) was used with optimum condition of 5% methanol mobile phase, 0.05% of nonafluorovaleric acid ion pairing reagent. Five standard Se species of Se(IV), Se(VI), SeCys(selenocystein), SeMet(selenomethionine) and Se-M-C(seleno methyl cystein) were separated successfully under the optimum condition (mobile phase; 5% methanol, ion-pairing reagent; 0.05% nonafluorovaleric acid, flow rate; 0.9 mL min?1 ). To extract Se species, microwave assisted and enzyme-assisted extraction methods were studied. In enzyme-assisted extraction method, protease I for garlic, protease I plus trypsin for pork and mackerel, and protease XIV for tuna showed the best extraction efficiency. With the optimum condition for each sample, it was found that mostly inorganic Se, SeCys and SeMet are present in the sample studied ranging from few μg g?1 to few tens of μg g?1.
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KEYWORD
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Ion exchange chromatography, RP HPLC, ICP-MS, HPLC-ICP-MS, Se species, seleno amino acids,
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